There are many enzymes that hydrolyze peptide bonds. They represent perhaps the most studied class of enzyme. the use of fluorogenic substrates has been very well documented. the most logical starting point to configure a protease assay is the identification of a suitable substrate. There are many commercially available solutions we will try to list most of them:

See also:

Peptides

University of Tour (France)		A list of selected synthetic substrates for various proteases
		protease and protease inhibitor Web server

AnaSpec		Generic Protease Assay Kits, Caspase Assay Kits, CMV/HIV/HCV Protease Assay Kits, MMP assay kits
Peptides International		Product list
Invitrogen		Detecting Peptidases and Proteases
DiscoverX		Products
Fluka		Fluorescent substrates to determine the activity of diverse Enzymes
Biotium		Fluorogenic peptidase and protease substrates that are based on : rhodamine, AFC, AMC or MCA.